OD085 - Biofilmes Orais: Aspectos Microbiológicos e Estruturais - 2S/2020 Imprimir

Ementa:

Formação e estrutura dos biofilme orais; Características dos biofilmes orais; Diferenças no crescimento microbiano em biofilme x planctônico; Composição microbiana dos biofilmes orais e ecologia do melo; Composição da matriz extracelular em biofilmes; Expressão gênica em biofilmes; Métodos de estudo de biofilmes orais.; Técnicas em biologia molecular (PCR, RT-qPCR, etc) para estudo de biofilmes; Técnicas de microscopia para estudo de biofilmes.

Bibliografia:

1. Amaechi BT, Tenuta LMA, Ricomini Filho AP, Cury JA. Protocols to Study Dental Caries In Vitro: Microbial Caries Models. Methods Mol Biol. 2019;1922:357-368. 2. Spinola MS, Nóbrega DF, Del Bel Cury AA, Ricomini Filho AP, Cury JA, Tenuta LMA. Fluoride Penetration and Clearance Are Higher in Exopolysaccharide-Containing Bacterial Pellets. Caries Res. 2019;53(1):16-23. 3. Nóbrega DF, Leitão TJ, Cury JA, Tenuta LMA. Fluoride Binding to Dental Biofilm Bacteria: Synergistic Effect with Calcium Questioned. Caries Res. 2019;53(1):10-15. 4. Lima CV, Tenuta LMA, Cury JA. Fluoride Increase in Saliva and Dental Biofilm due to a Meal Prepared with Fluoridated Water or Salt: A Crossover Clinical Study. Caries Res. 2019;53(1):41-48. 5. Fernández CE, Tenuta LMA, Del Bel Cury AA, Nóbrega DF, Cury JA. Effect of 5,000 ppm Fluoride Dentifrice or 1,100 ppm Fluoride Dentifrice Combined with Acidulated Phosphate Fluoride on Caries Lesion Inhibition and Repair. Caries Res. 2017;51(3):179-187. 6. Fernández CE, Tenuta LM, Cury JA. Validation of a Cariogenic Biofilm Model to Evaluate the Effect of Fluoride on Enamel and Root Dentine Demineralization. PLoS One. 2016 Jan 5;11(1):e0146478. 7. Ccahuana-Vásquez RA, Cury JA. S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization. Braz Oral Res. 2010 Jun;24(2):135-41. 8. Vogel GL, Tenuta LM, Schumacher GE, Chow LC. No calcium-fluoride-like deposits detected in plaque shortly after a sodium fluoride mouthrinse. Caries Res 2010;44:108-115. 9. Tenuta LM, Zamataro CB, Del Bel Cury AA, Tabchoury CP, Cury JA. Mechanism of fluoride dentifrice effect on enamel demineralization. Caries Res. 2009;43(4):278-285. 10. Aires CP, Del Bel Cury, Tenuta LM, Klein MI, Koo H, Duarte S, Cury JA. Effect of starch and sucrose on dental biofilm formation and on root dentine demineralization. Caries Res 2008;42:380-386. 11. Ccahuana-Vásquez RA, Vale GC, Tenuta LMA, Del Bel Cury AA, Vale GC, Cury JA. Effect of frequency of sucrose exposure on dental biofilm composition and enamel demineralization in the presence of fluoride. Caries Res 2007;41(1):9-15. 12. Tenuta LM, Del Bel Cury AA, Bortolin MC, Vogel GL, Cury JA. Ca, Pi and F in the fluid of biofilm formed under sucrose. J Dent Res 2006;85:834-8. 13. Ribeiro CCC, Tabchoury CPM, Del Bel Cury AA, Tenuta LMA, Rosalen PL, Cury JA. Composition and cariogenicity of dental biofilm formed in situ in the presence of starch and sucrose. Brit J Nutrition 2005;94(1):44-50. 14. Cury JA, Rebelo MA, Del Bel Cury AA, Derbyshire MT, Tabchoury CP. Biochemical composition and cariogenicity of dental plaque formed in the presence of sucrose or glucose and fructose. Caries Res 2000;34:491-7. 15. Vogel GL, Zhang Z, Chow LC, Carey CM, Schumacher GE, Banting DW. Effect of in vitro acidification on plaque fluid composition with and without a NaF or a controlled-release fluoride rinse. J Dent Res 2000;79(4):983-90. 16. Cury JA, Rebello MA, Del Bel Cury AA: In situ relationship between sucrose exposure and the composition of dental plaque. Caries Res 1997;31: 356-360. 17. Vogel GL, Mao Y, Carey CM, Chow LC. Increased overnight fluoride concentrations in saliva, plaque, and plaque fluid after a novel two-solution rinse. J Dent Res 1997;76:761-7. 18. Vogel GL, Chow LC, Brown WE. A microanalytical procedure for the determination of calcium, phosphate and fluoride in enamel biopsy samples. Caries Res 1983;17:23-31.

Ano de Catálogo: 2020

Créditos: 9